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. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: Virology. 2011 Dec 5;422(2):393–401. doi: 10.1016/j.virol.2011.11.004

Figure 2. dNTP concentration dependent reverse transcription activity of HIV-1 RT variants.

Figure 2

Steady-state incorporation rates of dNTPs by WT RT or A114 mutants were measured by using a 5′ end 32P-labled 23-mer A primer (P) annealed to a 38-mer RNA template was extended by HIV-1 RT proteins, (A) WT A114, (B) A114S, (C) A114C, (D) A114V, and (E) A114I showing ∼50% of primer extension with 250μM dNTPs (lane 1) at 37°C for 5 min. The reactions were repeated with decreasing dNTP concentrations (lanes 1-8: 100, 25, 5, 1, 0.5, 0.25, and 0.1 μM). F: 38 nt fully extended products. “*”: Lane 4 with 5μM dNTP.