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. Author manuscript; available in PMC: 2013 Oct 21.

Published in final edited form as: Virology. 2011 Dec 5;422(2):393–401. doi: 10.1016/j.virol.2011.11.004

Table 2.

Pre-steadv-state kinetic values of WT and A114C RT proteins with four dNTPs.

Enzyme	dNTPs	k_pol(sec^-1)	K_d (μM) (Fold difference)	k_pol/K_d (μM^-1sec¹)
WT	dTTP	85.7 ±7.3	3.4 ±0.6	25.2
	dGTP	39.8 ±1.4	1.7 ± 0.3	23.4.8
	dCTP	138.8 ±12.3	0.6 ±0.3	231.3
	dATP	69.6 ±5.4	1.2 ±0.4	58.0
	acyTTP	14.12 ± 1.2	6.9 ±2.7	2.1
A114C	dTTP	242.3 ±41.1	8.4 ±4.0 (2.5×)	28.8
	dGTP	33.3 ±2.9	8.1 ±2.1 (4.8×)	4.1
	dCTP	261.2 ±18.9	2.1 ±0.6 (3.5×)	124.3
	dATP	50.5 ±8.5	4.3 ±2.1 (3.6×)	11.7
	acyTTP	18.2 ±1.6 (1.3×)	5.7 ±1.4 (1.2×)	3.2