Table 2.
Enzyme | dNTPs | kpol(sec-1) | Kd (μM) (Fold difference) | kpol/Kd (μM-1sec1) |
WT | dTTP | 85.7 ±7.3 | 3.4 ±0.6 | 25.2 |
dGTP | 39.8 ±1.4 | 1.7 ± 0.3 | 23.4.8 | |
dCTP | 138.8 ±12.3 | 0.6 ±0.3 | 231.3 | |
dATP | 69.6 ±5.4 | 1.2 ±0.4 | 58.0 | |
acyTTP | 14.12 ± 1.2 | 6.9 ±2.7 | 2.1 | |
A114C | dTTP | 242.3 ±41.1 | 8.4 ±4.0 (2.5×) | 28.8 |
dGTP | 33.3 ±2.9 | 8.1 ±2.1 (4.8×) | 4.1 | |
dCTP | 261.2 ±18.9 | 2.1 ±0.6 (3.5×) | 124.3 | |
dATP | 50.5 ±8.5 | 4.3 ±2.1 (3.6×) | 11.7 | |
acyTTP | 18.2 ±1.6 (1.3×) | 5.7 ±1.4 (1.2×) | 3.2 |