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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Stem Cells. 2013 Aug;31(8):1644–1656. doi: 10.1002/stem.1412

Figure 2. Characterization of c-kit+/Lin− cells.

Figure 2

(A) Representative FACS analyses of c-kit, OCT4, SOX2, KLF4, and C-MYC. Negative control was obtained by omitting the primary antibodies.

(B) C-kit+ cells in culture express OCT4, SOX2, KLF4, C-MYC, nestin, NF-H, Acta2, vWF, isolectin, vimentin, ZO-1, NKCC2, AQP1, and NCCT. CD45 and β-3 tubulin are negative in C-kit+ cells.

(C) Gene expression by real-time PCR analyzed by the fold change (2ΔΔCt) in undifferentiated c-kit+ cells over neonatal rat kidney. Error bars represent mean ± SEM.

Cell nuclei are stained blue with DAPI. Scale bars represent 20 μm. Abbreviations: octamer-binding transcription factor (Oct3/4); sex-determining-region Y-box 2 (Sox2); Kruppel-like factor 4 (Klf4), neurofilament heavy chain (NF-H), alpha-actin 2 (Acta2), von Willebrand factor (vWF), zonula occludens-1 (ZO-1), Na-K-2Cl co-transporter (NKCC2), aquaporin-1 (AQP1), and Na-Cl co-transporter (NCCT).