Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Proteomics Clin Appl. 2013 May 21;7(0):355–366. doi: 10.1002/prca.201200069

Figure 1. Workflow employed for the analysis of gastric cancer secretome.

Figure 1

SILAC was employed to identify the more abundant proteins in the secretome of gastric cancer cells compared to the normal cells. Gastric cancer cell lines (AGS, SNU-1, SNU-1, SNU-5, SNU-16, NCI-N87, AGS and KATO-III) were grown in medium containing normal arginine and lysine. The normal gastric epithelial cell line was grown in a medium containing heavy arginine and lysine. Equal amounts of protein from pooled tumor secretome and from normal secretome were mixed. Proteins were subjected to fractionation by SDS-PAGE and strong cation exchange chromatography. The fractionated proteins were subjected to LC-MS/MS analysis using an LTQ-Orbitrap Velos mass spectrometer. The data was searched and quantitated using the Proteome Discoverer software suite.