FIG. 2.

Age reduced efficiency of thioredoxin (Trx) system in the myocardium. (A and B) Both Trx activity and thioredoxin reductase (TrxR) activity were significantly reduced in the aging rat heart either before or after myocardial ischemia/reperfusion (MI/R). (C) Age-dependent differences in TrxR1 protein expression. (D) Age-dependent differences in TrxR2 protein expression. Densitometry analysis for expression of TrxR1 and TrxR2 protein by western blot (n=8 each). Bar heights represent mean values and brackets indicate standard error of the mean (SEM). (*) p<0.05, (**) p<0.01 vs. Sham; (#) p<0.05 vs. Young. SY, Sham young; SA, Sham aged; RY, reperfused young; RA, reperfused aged.