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Aging enhances nitrative stress in the heart subjected to myocardial ischemia/reperfusion (MI/R). (A) Total NOx production was increased in the aging heart. (B) Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP) was reduced in heart tissues isolated from aged rat. (C) Nitrotyrosine content, as a marker for ONOO−, was increased in aging rat heart either before or after MI/R. (*) p<0.05, (**) p<0.01 vs. Sham; (#) p<0.05, (##) p<0.01 vs. Young.
