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. Author manuscript; available in PMC: 2013 Oct 21.
Published in final edited form as: J Endocrinol. 2013 Apr 15;217(2):175–184. doi: 10.1530/JOE-12-0559

Figure 3. Insulin-induced phosphorylation of AKT was not affected by acute psychological stress in skeletal muscle and adipose tissue.

Figure 3

At the end of the behavioral test, 5U/kg insulin was injected and skeletal muscle and adipose tissue were removed 20 min after injection. Tissue lysates, 30 μg per lane, were subjected to Western blotting with antibodies specific for PS473-AKT or total ERK. Representative Western blots are presented in A and C. (B and D) Autoradiographs were quantified by scanning densitometry and the data are presented as the mean ± S.E of 10 mice in IES with escape failure group (IES+EF group) and 10 mice in sham group. The phosphorylated protein level in the sham group was arbitrarily set to 100%. White gap in A and C indicates grouping of lanes from different parts of the same gel.