Figure 4. Integrative flow chart for discovery of knowledge to personalize DCIS treatment therapy.
The DCIS samples for NGS may come from experimental models or clinical samples (FFPE or fresh biopsy). Genes with similar expression patterns could be identified by RNA-Seq and the common promoter frameworks verified by ChIP-Seq. Combining systems bionetwork modeling approaches with clinicopathological characteristics, molecular genotype and phenotype, NGS results will help to discover / predict personalized therapy for DCIS patients.