Table 1. In silico ubiquitin machinery of medically relevant arthropod vectors when compared to known protein datasets* .
| Domains/Genomics | I. scapularis | An. gambiae | Ae. aegypti | C. quinquefasciatus | P. humanus | R. prolixus | S. cerevisiae | D. melanogaster | H. sapiens | M. musculus |
|---|---|---|---|---|---|---|---|---|---|---|
| Ubiquitin and ubiquitin-like proteins | ||||||||||
| APG12 | 2 | 3 | 2 | 2 | 4 | 2 | 1 | 3 | 1 | 1 |
| Atg8 (MAP1_LC3) | 3 | 6 | 2 | 3 | 3 | 2 | 1 | 2 | 15 | 8 |
| Ubiquitin | 31 | 40 | 48 | 40 | 35 | 129 | 7 | 30 | 45 | 41 |
| Ufm1 (UPF0185) | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 1 |
| Urm1 | 0 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 3 | 1 |
| Ubiquitin activating enzymes (E1) | ||||||||||
| ThiF/UBACT | 16 | 29 | 37 | 30 | 19 | 26 | 8 | 30 | 42 | 25 |
| Ubiquitin conjugating enzymes (E2) | ||||||||||
| UQ_con | 32 | 32 | 36 | 28 | 29 | 31 | 14 | 49 | 87 | 67 |
| Ubiquitin ligases (E3) | ||||||||||
| RING | 151 | 147 | 161 | 156 | 127 | 86 | 43 | 309 | 631 | 501 |
| HECT | 9 | 17 | 17 | 23 | 14 | 16 | 5 | 20 | 45 | 34 |
| Cullin | 5 | 6 | 9 | 4 | 7 | 8 | 4 | 14 | 16 | 11 |
| U-Box | 8 | 10 | 10 | 12 | 7 | 8 | 2 | 11 | 20 | 18 |
| F-Box | 43 | 39 | 49 | 151 | 32 | 39 | 12 | 46 | 111 | 104 |
| De-ubiquitinases | ||||||||||
| OTU | 7 | 9 | 9 | 9 | 7 | 6 | 2 | 12 | 21 | 18 |
| Josephin | 2 | 1 | 1 | 2 | 2 | 2 | 0 | 1 | 13 | 10 |
| JAB (Mov34) | 12 | 10 | 11 | 18 | 10 | 8 | 4 | 11 | 17 | 8 |
| DUF862 | 3 | 1 | 2 | 3 | 2 | 11 | 0 | 5 | 8 | 2 |
| WLM | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 |
| UCH | 25 | 29 | 28 | 34 | 26 | 29 | 18 | 50 | 123 | 86 |
| Peptidase_C12 | 4 | 2 | 7 | 3 | 4 | 3 | 1 | 6 | 7 | 6 |
| Peptidase_C48 | 8 | 3 | 4 | 3 | 5 | 4 | 2 | 10 | 16 | 12 |
| Peptidase_C54 | 2 | 3 | 4 | 4 | 1 | 2 | 1 | 2 | 7 | 5 |
| Total | 366 | 392 | 439 | 528 | 337 | 415 | 127 | 614 | 1229 | 959 |
Protein datasets were downloaded from online databases and matched with selected Pfam domains using the search program of the HMMER 3.0 package. Search results are listed individually.