Table 1. Summary Information on Eighteen Sequenced Small RNA Libraries from a Developmental Series of Williams (PI548631) Seed and Seed Coats Including Developmental Stage and Number of Reads.
Tissue | Developmental stage | Replicate | Instrument model | # of Reads |
Whole Seed | 4 days after flowering | BR1 | Illumina HiSeq 2000 | 27.0 M |
Whole Seed | 4 days after flowering | BR2 | Illumina HiSeq 2000 | 12.1 M |
Whole Seed | 12–14 days after flowering | BR1 | Illumina HiSeq 2000 | 35.2 M |
Whole Seed | 12–14 days after flowering | BR2 | Illumina HiSeq 2000 | 13.1 M |
Whole Seed | 22–24 days after flowering | BR1 | Illumina HiSeq 2000 | 30.8 M |
Whole Seed | 22–24 days after flowering | BR2 | Illumina HiSeq 2000 | 10.3 M |
Seed Coat | 5–6 mg fresh weight | BR1 | Illumina HiSeq 2000 | 17.8 M |
Seed Coat | 5–6 mg fresh weight | BR2 | Illumina HiSeq 2000 | 25.3 M |
Seed Coat | 10–25 mg fresh weight | NA | Illumina HiSeq 2000 | 12.0 M |
Seed Coat | 25–50 mg fresh weight | NA | Illumina HiSeq 2000 | 12.0 M |
Seed Coat | 50–75 mg fresh weight | BR1 | Illumina Genetic Analyzer | *2.9 M |
Seed Coat | 50–75 mg fresh weight | BR2 | Illumina HiSeq 2000 | 11.5 M |
Seed Coat | 75–100 mg fresh weight | BR1 | Illumina Genome Analyzer II | 30.1 M |
Seed Coat | 75–100 mg fresh weight | BR2 | Illumina HiSeq 2000 | 10.5 M |
Seed Coat | 200–300 mg fresh weight | BR1 | Illumina HiSeq 2000 | 31.3 M |
Seed Coat | 200–300 mg fresh weight | BR2 | Illumina HiSeq 2000 | 14.8 M |
Seed Coat | 300–400 mg fresh weight | BR1 | Illumina Genome Analyzer II | 34.0 M |
Seed Coat | 300–400 mg fresh weight | BR2 | Illumina HiSeq 2000 | 17.1 M |
The PI number is an accession number by which information on the cultivar is searchable in the USDA GRIN (Germplasm Resources Information Network). Williams is homozygous dominant ii genotype with yellow seed coats.
This library was previously reported in [4]. BR1 and BR2 are biological repeats; NA, not applicable.