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. 2013 Oct 21;8(10):e79464. doi: 10.1371/journal.pone.0079464

Figure 2. N. crassa SIN components localize to SPBs and septa (A).

Figure 2

Functional GFP fusion proteins of CDC-7, SID-1, CDC-14 and DBF-2 localized to spindle pole bodies (arrows) and as constricting rings at forming septa. Nuclei were labeled with histone H1-RFP, the cell wall was stained with Calcofluor White. (B) The localization of the three SIN kinases CDC-7, SID-1 and DBF-2 to SPBs is constitutive and cell cycle independent. The three SIN kinases associate with SPBs of interphase nuclei as well as during early and late mitotic stages (as indicated by nuclear morphology). Nuclei were labeled with histone H1-RFP.