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. 1972 May;23(5):841–845. doi: 10.1128/am.23.5.841-845.1972

Preparation of Purified Suspensions of Coxiella burneti by Genetron Extraction Followed by Continuous-Flow Ultracentrifugation1

Doria R Dubois 1,2, Ernest C Cutchins 1,2, Sanford Berman 1,2, Joseph P Lowenthal 1,2, Robert L Timchak 1,2
PMCID: PMC380458  PMID: 4555634

Abstract

A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altieri P. L., Berman S., Groffinger A., Lowenthal J. P. Preparation of cholera vaccines by fluorocarbon extraction of cholera Vibrio suspensions. Infect Immun. 1970 Apr;1(4):334–337. doi: 10.1128/iai.1.4.334-337.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson N. G., Barringer H. P., Amburgey J. W., Jr, Cline G. B., Nunley C. E., Berman A. S. Continuous-flow centrifugation combined with isopycnic banding: rotors B-8 and B-IX. Natl Cancer Inst Monogr. 1966 Jun;21:199–216. [PubMed] [Google Scholar]
  3. Berman S., Gochenour R. B., Cole G., Lowenthal J. P., Benenson A. S. METHOD FOR THE PRODUCTION OF A PURIFIED DRY Q FEVER VACCINE. J Bacteriol. 1961 May;81(5):794–799. doi: 10.1128/jb.81.5.794-799.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cline G. B., Nunley C. E., Anderson N. G. Improved continuous flow centrifugation with banding. Nature. 1966 Oct 29;212(5061):487–489. doi: 10.1038/212487a0. [DOI] [PubMed] [Google Scholar]
  5. Elliott J. A., Ryan W. L. Continuous-flow ultracentrifugation of canine distemper virus and infectious canine hepatitis virus. Appl Microbiol. 1970 Nov;20(5):667–671. doi: 10.1128/am.20.5.667-671.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fox S. M., Birnie G. D., Martin E. M., Sonnabend J. A. Isolation of polyoma virus and Semliki Forest virus from tissue culture fluid by continuous flow zonal ultracentrifugation. J Gen Virol. 1967 Oct;1(4):577–580. doi: 10.1099/0022-1317-1-4-577. [DOI] [PubMed] [Google Scholar]
  7. Gerin J. L., Richter W. R., Fenters J. D., Holper J. C. Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses. J Virol. 1968 Sep;2(9):937–943. doi: 10.1128/jvi.2.9.937-943.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KENT J. F., FIFE E. H., Jr Precise standardization of reagents for complement fixation. Am J Trop Med Hyg. 1963 Jan;12:103–116. doi: 10.4269/ajtmh.1963.12.103. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. ORMSBEE R. A. A method of purifying Coxiella burnetii and other pathogenic Rickettsiae. J Immunol. 1962 Jan;88:100–108. [PubMed] [Google Scholar]
  11. Reimer C. B., Baker R. S., Van Frank R. M., Newlin T. E., Cline G. B., Anderson N. G. Purification of large quantities of influenza virus by density gradient centrifugation. J Virol. 1967 Dec;1(6):1207–1216. doi: 10.1128/jvi.1.6.1207-1216.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STOKER M. G. Variation in complement-fixing activity of Rickettsia burneti during egg adaptation. J Hyg (Lond) 1953 Sep;51(3):311–321. doi: 10.1017/s0022172400015746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spicer D. S., DeSanctis A. N., Beiler J. M. Preparation of highly purified concentrates of Coxiella burneti. Proc Soc Exp Biol Med. 1970 Dec;135(3):706–708. doi: 10.3181/00379727-135-35125. [DOI] [PubMed] [Google Scholar]

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