Figure 4. Effect of increasing CBD concentrations on ERK phosphorylation in U87-MG and T98G glioma cells.

U87-MG and T98G cells were treated with CBD for 24 h and lysates from cells were used to assess ERK protein levels. (A,C) Western blot analysis of phospho- and total-ERK. A representative western blot is shown. (B,D) Densitometric analysis of ERK signal bands from three independent experiments is reported. *p<0.05, **p<0.01 vs Control [C], Dunnett’s t test.