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. 2013 Oct 21;8(10):e77291. doi: 10.1371/journal.pone.0077291

Figure 2. Expression system for dandelion PPOs. A Scheme for the PPO-2 expression plasmid.

Figure 2

Location of important genes, regulatory elements and sites for restriction enzymes are given. The StrepII-tag (WSHPQFEK) and the Enterokinase recognition site (Ek-site: DDDDK) were introduced via PCR. ORF: open reading frame. B Procedure of heterologous PPO-expression. Conditions like media, supplements and temperatures are given and the course of action is schematized. AIM: autoinduction medium; LB: lysogeny broth.