Skip to main content
. 2013 Oct 21;8(10):e77291. doi: 10.1371/journal.pone.0077291

Figure 4. Optimal time for harvest of recombinant ToPPO-2. A SDS-PAGE of crude protein extracts.

Figure 4

Samples were taken at different sampling times and analyzed by SDS-PAGE (same total protein amount per well). To visualize protein composition a Coomassie-staining was performed. B Western blot analysis. Proteins were blotted on a nitrocellulose membrane. Strep-Tactin-HRP conjugate was used to specifically detect the StrepII-tag of the recombinant PPO. C Bacterial growth and PPO-activity over sampling time. Samples were checked for their OD at 600 nm. Additionally crude protein extracts were analyzed for PPO-activity per mg of total protein. The presented data show one experiment representative for three replicates.