Figure 6. Dependency of PPO-expression on the amount of copper.

Production cultures were grown with different amounts of CuCl₂ supplemented in the AIM. A SDS-PAGE of crude protein extracts. Samples were taken after 48 h and analyzed by SDS-PAGE (equal total protein amount per well). To visualize protein composition a Coomassie-staining was performed. B Western blot analysis. Proteins were blotted on a nitrocellulose membrane. StrepTactin-HRP-conjugate was used to specifically detect the StrepII-tag of the recombinant PPO. C Bacterial growth and PPO-activity. Samples were checked for their OD at 600 nm. The average value from all samples is given as a dotted line. Additionally, crude protein extracts were analyzed for PPO-activity per mg of total protein.