Table 1. Nucleotide sequences of the primers used to amplify the cDNA and the genomic DNA of the OitaAP2 locus of Orchis italica.
Name | Direction | Sequence (5′-3′) | Position |
AP2F8 | F | ATGGTGCTAGATCTCAACGTGTCAT | 1–25 |
AP2F4new | F | CATCAAACTCCTCTGTTCTCAATG | 89–112 |
AP2F1 | F | GATGGGARTCKCAYATYTGGGA | 509–530 |
AP2F6 | F | GGAAGAATTTGTGCATATTCTTCGG | 690–714 |
AP2F5 | F | CTTCACAAATGTGGGCGGTG | 766–785 |
AP2R5 | R | CACCGCCCACATTTGTGAAG | 785–766 |
AP2F2 | F | TGGGARGCTMGNATGGGNCARTT | 780–806 |
AP2RealF_iso | F | CAAGAAATTGAAGGAAAGGGCCATGG | 1089–1101; 1207–1219 |
AP2F4old | F | GAGCATCCTCATGTTTGGGGCAG | 1153–1175 |
AP2R4old | R | CTGCCCCAAACATGAGGATGCTC | 1175–1153 |
AP2RealF | F | TGTGTACCCCGGATTATTTCCT | 1176–1197 |
AP2R2 | R | AGGAAATAATCCGGGGTACACA | 1197–1176 |
AP2R1 | R | TTTCTGGGGCCAAGTGGTCATGGT | 1260–1237 |
APF3 | F | TGCAGCATCATCAGGATTC | 1296–1314 |
APR3 | R | GAATCCTGATGATGCTGCA | 1314–1296 |
AP2R9 | R | TCAGCTCTGAAAGAAGTGATGACG | 1431–1407 |
AP2R4 | R | CCTCTGGCTTCATTTGATATTGAG | 1558–1535 (5′-UTR) |
The primer positions are numbered starting from the first base of the ATG start codon (position 1) of the OitaAP2 cDNA.