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. 2013 Oct 21;8(10):e77454. doi: 10.1371/journal.pone.0077454

Table 1. Nucleotide sequences of the primers used to amplify the cDNA and the genomic DNA of the OitaAP2 locus of Orchis italica.

Name Direction Sequence (5′-3′) Position
AP2F8 F ATGGTGCTAGATCTCAACGTGTCAT 1–25
AP2F4new F CATCAAACTCCTCTGTTCTCAATG 89–112
AP2F1 F GATGGGARTCKCAYATYTGGGA 509–530
AP2F6 F GGAAGAATTTGTGCATATTCTTCGG 690–714
AP2F5 F CTTCACAAATGTGGGCGGTG 766–785
AP2R5 R CACCGCCCACATTTGTGAAG 785–766
AP2F2 F TGGGARGCTMGNATGGGNCARTT 780–806
AP2RealF_iso F CAAGAAATTGAAGGAAAGGGCCATGG 1089–1101; 1207–1219
AP2F4old F GAGCATCCTCATGTTTGGGGCAG 1153–1175
AP2R4old R CTGCCCCAAACATGAGGATGCTC 1175–1153
AP2RealF F TGTGTACCCCGGATTATTTCCT 1176–1197
AP2R2 R AGGAAATAATCCGGGGTACACA 1197–1176
AP2R1 R TTTCTGGGGCCAAGTGGTCATGGT 1260–1237
APF3 F TGCAGCATCATCAGGATTC 1296–1314
APR3 R GAATCCTGATGATGCTGCA 1314–1296
AP2R9 R TCAGCTCTGAAAGAAGTGATGACG 1431–1407
AP2R4 R CCTCTGGCTTCATTTGATATTGAG 1558–1535 (5′-UTR)

The primer positions are numbered starting from the first base of the ATG start codon (position 1) of the OitaAP2 cDNA.