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. 2013 Oct 21;8(10):e77784. doi: 10.1371/journal.pone.0077784

Figure 1. Developmental classification of hESC-VCMs.

Figure 1

A) A representative FACS plot showing that approximately 55% of hESC-derivatives were positive for cardiac troponin-T (cTnT) prior to selection. B) Selected MLC2V-mCherry+ hESC-VCMs (~95% purified) under brightfield and fluorescence microscopy (100x). C) Purified HESC-VCMs were stained with anti-cTnT and anti-α-actinin antibodies. D) Representative action potential of hESC-VCMs. E) Hierarchical clustering showing that biological replicates cluster together. Red and green indicates up- and down-regulation respectively. F) Principal Component Analysis. HESC-VCMs, hF-VCMs and hA-VCMs lie along a linear developmental axis (dotted black line). Correlation coefficient is shown to indicate the degree of linearity. G) An activation isochronal map of trans-membrane potential with 15ms intervals. H) CX43 staining of hESC-VCMs. Red= CX43 staining, blue=DAPI.