Figure 5.

TGFβ-induced anchorage-independent growth (AIG) and migration require PDGFR and ErbB mediation. A) Analyses of TGFβ1-stimulated AIG in the presence of lapatinib and/or imatinib. Treatments were done in triplicate; number of experiments included for each particular treatment is indicated. Error bars = sem. Significant inhibition of ErbB and PDGFR can be attained using higher concentrations of lapatinib and imatinib, respectively, whereas a combination of both drugs at suboptimal doses yields a synergistic effect. B) Analyses of TGFβ1-induced cell migration in the presence of pharmacological inhibitors of ErbB (lapatinib; 5 μM), PDGFR (AG1295; 20 μM), and MEK (U0126; 10 μM). Representative photomicrographs are shown. C) Quantitative analysis of B, where values are from 2 independent experiments, with 4 replicates/treatment. Error bars = sd. Results in B and C show that all 3 pathways are required for TGFβ-induced cell migration.