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. 2013 Sep;16(9):973–978.

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Competition assay. One group of cultured K562 cells was treated with ferrous citrate in transferrin solution 15 min prior to transfection with OEI-HA10/Tf-PEI/Luc-siRNA formulation and the other was not. Cells un-treated with transferrin solution, showed a significant suppression in luciferase activity, while this knocking down effect dropped significantly in the cells treated with transferrin solution. White bars represent luciferase activity (%) in comparison to untreated control cells (100%) obtained by using Luc-siRNA and gray bars represent activities obtained with siCONTROL. Data are mean and relative S.D. of 5 similar experiments. Asterisks indicate statistical significance comparing transferrin treated vs. untreated cells (*P<0.05; paired-sample t- test