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. 2013 Oct 21;210(11):2371–2385. doi: 10.1084/jem.20122348

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© 2013 Zhou et al.

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Figure 2. — RhoA deficiency reduces hematopoietic progenitors but not HSCs. (A) Frequency of CFCs in BM 5 d after poly I:C treatments. (B and C) Number of LK and LSK cells in BM 5 d after poly I:C treatments. (B) Representative flow cytometry plots of BM LK and LSK populations. (C) Quantification of LK and LSK cell numbers in tibia, femur, and iliac crest. (D and E) The number of Lin⁻SLAM cells in BM. (D) Representative flow cytometry plots of the Lin⁻SLAM population. (E) Quantification of the number of Lin⁻SLAM cells in tibia, femur, and iliac crest. All mice used in this figure were congenic transplantation recipients as described in Fig. 1 G. Numbers of samples analyzed: five per group (A and C) and four (RhoA^fl/fl) and five (RhoA-cKO; E). (A–E) The results from a representative experiment of two independent experiments are shown. Error bars indicate SEM. ***, P < 0.001.