Figure 3.

Transient T reg cell ablation is sufficient for inhibition of tumor growth without significant side effects. (A) Growth kinetics of orthotopically implanted tumors treated with 25 µg/kg DT at the indicated times; ****, P < 0.0001. Error bars represent SEM. (B) Number of metastatic nodules present on the lung surface upon examination under a dissection microscope; ***, P < 0.001. Error bars represent SD. (C) Body weight fluctuations represented as percentage of weight at the time of initial DT administration. Error bars represent SEM. (D) Representative histological images of liver, kidney, heart, and pancreas from control and DT-treated mice 2 wk after treatment. n = 3–5 mice per group, representative of at least three independent experiments. Bars, 50 µm. P-values were calculated using ANOVA followed by Bonferroni’s post-hoc test (A) and Student’s t test (B).