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. 2013 Oct 21;210(11):2351–2369. doi: 10.1084/jem.20122019

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© 2013 Le Bouteiller et al.

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Figure 9. — Rapid BM cytopenia is mediated by p53. (A, left) Representative histograms for p53 labeling in SLAM cells and MPPs (Lin⁻ Sca-1⁺ CD150⁺ CD48⁺) at day 4 after Cre induction from control and Nle^cKO mice. (right) Representative histograms for p53 labeling in BM pre-B and immature B cells from CD19-control and CD19-Nle^KO mice. (B) Quantification of p53 levels in populations from control and cKO mice. Bars are means (SD) of n = 4 mice per genotype. (C) Quantification of p53 levels in B cell populations from CD19-control and CD19-Nle^KO mice. Bars are means (SD) of n = 3 mice per genotype. Data are from one representative of two experiments. (D) Relative expression levels of p53 target genes measured by RT-QPCR in SLAM cells and MPPs from control and Nle^cKO mice at day 4 after Cre induction. Bars are means (SD) of n = 4 mice. Data are from one representative of two experiments. (E) Representative FACS profiles obtained 5 d after Cre induction from control, Nle^cKO, and Nle^cKO p53^−/− (Rosa26^CreERT2/+; Nle^Flox/null; p53^−/−) mice. The BM cellularity and the number of LSK cells are shown for the three genotypes. Bars are means (SD) for n = 4–6 mice per genotype. This experiment was performed twice. (F) Its2 labeling by FISH was performed on BM cells from control, Nle^cKO, and Nle^cKO p53^−/− mice 5 d after Cre induction. Representative histograms for the levels of its2 in SLAM cells and MPPs (Lin⁻ Sca-1⁺ CD150⁺ CD48⁺) and quantification of its2 intensity are shown. Bars are means (SD) for n = 4–6 mice per genotype. This experiment was performed twice. *, P < 0.05.