Figure 5.

Ex vivo LPS-stimulated mouse and human macrophages have increased TNF and IL-6 production in an age-dependent manner. (A) Young (4 mo) or aged (17–18 mo) C57BL/6 mice received PBS or LPS and on day 3, monocytes from the BM were isolated, differentiated into macrophages (BMDM), and ex vivo restimulated with either PBS or LPS for 24 h to assess TNF and IL-6 levels. (B–E) Monocytes derived from the PBMCs of 37 donors aging from 28 to 95 yr old were isolated and differentiated into macrophages over 10 d and were then stimulated for 48 h with 50 ng/ml LPS or PBS, and TNF and IL-6 production was assessed. Data are illustrated in two different manners: (B and C) data for TNF and IL-6 production by macrophages in donor cohorts ranging from 28 to 59 yr old as a scatter plot; (D and E) data analyzed by grouping the 28–59 and the 63–95 yr olds. Mouse data were analyzed by one-way analysis with Bonferroni’s post-tests, n = 3. A is representative three independent experiments and B–E are representative of one experiment. Human data in D and E were analyzed by a Student’s t test (horizontal bars represent means), and p-values are indicated on graphs in C and D.