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. 2013 Oct 21;210(11):2191–2204. doi: 10.1084/jem.20130028

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© 2013 Nakamura et al.

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Figure 10. — The expression of Bach2 in hematopoietic cells is essential for pulmonary homeostasis. (a) The protocol for the BM transplantation to examine the effects on PAP. The experiments were performed using three mice for each group. (b) The flow cytometric analysis of peripheral blood cells to confirm the success of the transplant. (c) The histological examination (HE staining) of the lungs 2 mo after BM transplantation. Bars, 100 µm. (d) The protocol for the BM transplantation to examine the therapeutic effects. Because Bach2-deficient mice over 16 wk of age had become weakened, we applied only 500 rads during the irradiation of these mice. The experiments were performed using two Bach2-deficient mice. (e) The flow cytometric analysis of the peripheral blood cells to confirm the transplantation. (f) The CT images of the lungs of Bach2-deficient mice before and after the transplantation. (g) The histological examination (HE staining) of the lungs 2 mo after the BM transplantation. Bars, 100 µm.