Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Oct 21;210(11):2305–2320. doi: 10.1084/jem.20130958

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Reantragoon et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — In situ localization and TCR repertoire analysis of jejunal MAIT cells. (A) Immunohistochemical staining of MAIT cells in the lamina propria in a human jejunal tissue section. Brown indicates TRAV1-2 (D5 mAb) staining detected with peroxidase-conjugated secondary antibody. The red arrow shows an example of a TRAV1-2⁺ intraepithelial MAIT cell stained with the D5 mAb. (B) Flow analysis of human IELs prepared from jejunal sections with mAbs against CD3, CD4, and CD161 and either human MR1-Ag tetramer (rRL-6-CH₂OH; top left) or empty MR1-tetramer (top right) or TRAV1-2–reactive mAb (Vα7.2; bottom left) or isotype control (bottom right). Tetramer or TRAV1-2 and CD161 staining are shown on the y and x axis, respectively. This staining experiment was performed three times with similar results. (C) Pie chart showing TRAJ usage of MAIT IELs (CD161^hiTRAV1-2⁺). Proportions are calculated from a total of 113 sequences.