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. Author manuscript; available in PMC: 2013 Oct 22.
Published in final edited form as: J Immunol. 2011 Feb 14;186(6):3563–3571. doi: 10.4049/jimmunol.1003933

Figure 3.

Figure 3

A, Phylogenetic tree analysis of Ring3 and homologues. GenBank accession numbers used in this analysis are as follows. Ring3 (BRD2): CAM25760 (human), AAY34703 (bovine), CAI11405 (dog), CAA15819 (mouse), CAE83937 (rat), XP_001369391 (opossum), CAN13285 (pig), CAA65449 (chicken), BAC82511 (quail), AAI68574 (X. tropicalis), AAI30180 (X. laevis), CAK04960 (zebrafish-1), CAD54663 (zebrafish-2), ABQ59684 (salmon), BAD93258 (medaka). Additional accession numbers for Ring3 homologues used for this analysis are the following: BRD3: AAI29055 (X. laevis), NP_031397 (human), NP_075825 (mouse), XP_001365890 (opossum), XP_425330 (Chicken). BRDT: NP_473395 (mouse), NP_997072 (human), XP_537079 (dog). BRD4: NP_490597 (human), NP_065254 (mouse), NP_001104751 (zebrafish), AAH76786 (X. laevis). BRD1: NP_001157300 (horse), XP_698063 (zebrafish), NP_001085846 (X. laevis), CAG30294 (human). Gene names are noted after species name. BRD1 does not map to an MHC paralogous region, whereas BRDT, BRD3, and BRD4 are found in the MHC paralogous regions. The tree was constructed using the NJ method, rooted with BRD1, and bootstrapping analysis was done with 1000 runs. Values are noted at the branch nodes, and an asterisk (*) indicates no significant value. The scale indicates the divergence time. B, The shark ring3 maps to the MHC. Primers from exons 4 and 5 were used for PCR amplification and ssCP analysis. The ∼1440-bp amplicon from the siblings along with mother shark genomic DNA were loaded on an 0.5× MDE gel. Under these conditions, “m2” was identified as two distinctive bands indicated as arrows. Mother and sibling numbers are indicated above the gel along with MHC groups and haplotype combinations from previous work (36).