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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Protein Expr Purif. 2013 Aug 1;91(2):119–124. doi: 10.1016/j.pep.2013.07.013

Figure 1.

Figure 1

SDS-PAGE analysis of KcsA during purification is shown. Panel A shows results from Nickle column on the lysate from pask90 expression and panel B shows results using the PQE-60 expression system. The band around 50 KDa is the KcsA tetramer. Weak bands at ~100 KDa and ~20 KDa correspond to octamers and monomers respectively. Panel A lanes: 1-marker 2-cell lysate supernatent, 3,4 – consecutive 50 mM imidazole washes, 5,6 - 100 mM imidazole elution 7– 150 mM imidazole elution, 8- 200 mM imidazole elution, 9- 250 mM imidazole elution, 10- pure KcsA standard. Panel B lanes: 1,3,4- 50 mM imidazole washes, 2-cell lysate supernatant, 5–6 – 100 mM imidazole elution, 7 – 150 mM imidazole elution, 8– 200 mM imidazole elution, 9–10 – 250mM imidazole elution.