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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Life Sci. 2013 Jul 17;93(8):313–322. doi: 10.1016/j.lfs.2013.07.005

Figure 2. Protein content of cardiolipin biosynthetic pathway constituents.

Figure 2

Assessment of cardiolipin biosynthetic pathway constituents was performed by Western blot using specific antibodies. (A) and (B): representative CDS content from SSM and IFM control (n=7) and diabetic (n=8), respectively; (C) and (D): representative PGS content from SSM (n=7 for each group) and IFM (n=8 for each group) control and diabetic, respectively; (E) and (F): representative CRLS content from SSM and IFM control (n=8) and diabetic (n=7), respectively; (G) and (H): representative TAZ content from SSM and IFM control (n=7) and diabetic (n=7), respectively. Control for loading was confirmed using COX IV probing. Values are expressed as means ± SEM; *P< 0.05 for control vs. diabetic groups. Abbreviations: CDS = cytidine diphosphate diacylglycerol synthase; PGS = phosphatidylglycerol synthase; CRLS = cardiolipin synthase; TAZ = tafazzin; IFM = interfibrillar mitochondria; SSM = subsarcolemmal mitochondria.