Table 5.
Proteins identified in 40 % or less of the mass spectrometric experiments conducted with the PAB1 deletion derivatives
| Protein | Mol. wt. (kDa) | Unique peptides (Avg.) | PAF score | Function related to PAB1 |
|---|---|---|---|---|
| GCN1 | 298 | 2.5 | 0.083 | Translation |
| RPB1 | 192 | 0.36 | 0.018 | Transcription |
| SKI2 | 147 | 0.64 | 0.044 | mRNA degradation |
| RPC82 | 74 | 1.2 | 0.16 | Transcription |
| UTP15 | 58 | 0.21 | 0.036 | Nucleolar |
| RPC34 | 36 | 0.28 | 0.077 | Transcription |
| RPB5 | 26 | 0.28 | 0.11 | Transcription |
| SLX9 | 25 | 0.43 | 0.17 | Unknown |
The proteins PRP8, POL2, yER138c, TOP2, and RAT1 were detected in several of the mass spectrometric experiments but were not included in the list because they were not detected in the wild-type pull-downs when the control experiments were conducted