Table 6.
Average number of unique peptides identified for proteins co-purifying with PAB1
| Protein | PAB1-wt | ΔRRM1 | ΔRRM2 | ΔRRM3 | ΔRRM4 | ΔP | ΔC |
|---|---|---|---|---|---|---|---|
| PAB1 | 66 | 68 | 70 | 66 | 68 | 60 | 70 |
| eIF4G1 | 50 | 20 | 16 | 46 | 46 | 42 | 42 |
| eIF4G2 | 25 | 10 | 7 | 24 | 20 | 10 | 24 |
| UPF1 | 26 | 8 | 22 | 21 | 22 | 18 | 24 |
| CBC1 | 18 | 13 | 17 | 12 | 7 | 2 | 16 |
| NAB6 | 14 | 6 | 10 | 12 | 20 | 18 | 17 |
| eRF3 | 14 | 8 | 20 | 14 | 8 | 2 | 6 |
| PBP2 | 12 | 14 | 10 | 11 | 11 | 7 | 0 |
| SSD1 | 10 | 16 | 11 | 4 | 13 | 8 | 10 |
| NOP77 | 10 | 5 | 13 | 8 | 6 | 9 | 10 |
| GBP2 | 10 | 4 | 6 | 14 | 10 | 12 | 16 |
| SLF1 | 8 | 2 | 5 | 4 | 3 | 3 | 8 |
| CBF5 | 4 | 4 | 2 | 4 | 0.5 | 2 | 4 |
| yGR250c | 3 | 6 | 13 | 6 | 12 | 0.5 | 6 |
Mass spectrometric analysis on proteins co-purifying with individual Flag-PAB1 variants was conducted as described in the text. Only those proteins that are listed in Table 4 were compared. Values refer to the average number of unique peptides detected for each protein for the two series of experiments that were conducted. Values in bold displayed significant differences (at least two-fold differences in values as compared to the wild type)