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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Anal Biochem. 2013 Jul 26;442(1):97–103. doi: 10.1016/j.ab.2013.07.022

Figure 1. Absolute quantitation of endogenous Erk2 in LNCaP Lysate.

Figure 1

Serial dilutions of GST-Erk2 recombinant proteins were spiked into 16.5 ng LNCaP lysate, recombinant protein / cell lysate samples were mixed with sample loading master mix and analyzed with the Simple Western system as described under Methods. (A) Pseudo-gel and (B) electro-pherogram image of Simple Western results using anti-Erk1/2 antibody for blotting; (C) GST-Erk2 signal intensity is plotted versus spiked-in amount of GST-Erk2. Detection linearity is observed with 1.25 to 40 pg GST-Erk2; (D) Linear regression analysis for endogenous Erk2 determination. 6.67 pg of GST-Erk2 was predicted from the best-fit linearity curve when intensity ratio = 1. Data are of a representative experiment. Dash-lines indicate the 95% confidential intervals.