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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Anal Biochem. 2013 Jul 26;442(1):97–103. doi: 10.1016/j.ab.2013.07.022

Figure 2. Matrix effect on Erk2 signal.

Figure 2

10 pg (Run 1& 3) or 20 pg (Run 2) of GST-Erk2 was spiked into HeLa, MCF-7, and LNCaP cell lysates prepared with M-per or RIPA buffer and analyzed with the Simple Western system. (A) Pseudo-gel image of one representative run indicates almost identical GST-ERk2 signal strength in different cell lysates prepared with either M-per or RIPA buffer. (B) GST-Erk2 signals were normalized to the GST-Erk2 signal in HeLa M-per lysate and compared.