FIGURE 4.
DAD regulation of LP Ih is necessary for phase recovery in 5 μM. Plots of fold-changes in LP-on phase over time for dynamic clamp (solid lines) and control (dashed line) experiments indicate that introduction of a dynamic clamp current to abrogate DAD regulation of LP Ih prevents phase recovery; thin lines, individual experiments with dynamic clamp (n = 5); thick line, average for experiments with dynamic clamp; dashed line, control experiment that was exactly the same as the dynamic clamp experiments except that the dynamic clamp was turned off during the 1 h superfusion with 5 μM DA. Repeated measures ANOVA with Dunnett’s post hoc tests that compared all time points to t = 0 showed that average LP-on phase did not recover in experimental preparations, F(6,4) = 16.04, p < 0.0001; *p < 0.05. Note that phase did recover in the control experiment.