Fig. 1.

a Cardiac specific β-TM construct. β-TM cDNA coding for amino acids (aa) 1–284 and the 3′ untranslated region (UTR) of β-TM was linked to the α-myosin heavy chain promoter at the 5′ end and to the SV40 polyadenylation cassette at the 3′ end. b Cardiac specific γ-TM construct. γ-TM cDNA coding for amino acids (aa) 1–284 and the 3′ untranslated region (UTR) of γ-TM was linked to the α-myosin heavy chain promoter at the 5′ end and to the HGH polyadenylation cassette at the 3′ end. c Genomic Southern blot analysis of NTG, β-TM, γ-TM and βγ-DTG mice. Mouse-tail genomic DNA was digested with EcoRI, Southern blotted, and hybridized to ³²P-radiolabeled β-TM or γ-TM 3′-UTR probes. d RNA expression in transgenic mice. Ten micrograms of total RNA (pooled from five mice for each line) were electrophoresed, transferred to nitrocellulose membrane, and probed with ³²P-radiolabeled β-TM 3′-UTR, γ-TM 3′-UTR, α-TM 5′-UTR, or GAPDH