FIG. 1.

Properties of the clonal cell line m17.ASC. Morphology of the m17.ASC clone, which was generated from Sca-1+cell cultures plated at low density (200 cells/cm²) at the phase-contrast microscope at two different enlargements (a). Phenotype of m17.ASCs, characterized in RT-PCR (b) and cytofluorimetry (c, d) for different stemness markers at different passages. The clone has a fibroblast-like aspect and continues to express stemness markers with time. Representative experiments out of the three performed are reported. SVF, cells from stromal vascular fraction; ASC, adipose derived stem cells.