Table 1.

Primers Used for the DNA Amplification of Specific Target Genes

Target gene	Forward sequence	Reverse sequence	Fragment size (bp)	Annealing (°C)
Sca-1	5′-ACTGTGCCTGCAACCTTGTCTGAGA-3′	5′-GTCCAGGTGCTGCCTCCATT-3′	322	62
c-kit	5′-GCCCTAATGTCGGAACTGAA-3′	5′-TTGCGGATCTCCTCTTGTCT-3′	319	60
Nucleostemin	5′-GGGAAAAGCAGTGTCATTA-3′	5′-GGGATGGCAATAGTAACC-3′	405	54
Islet-1	5′-GCCTCAGTCCCAGAGTCATC-3′	5′-AGAGCCTGGTCCTCCTTCTG-3′	308	60
Nestin	5′-TCAAGGGGAGGCCAGGAAGGA-3′	5′-CTGCAGCCCCACTCAAGCCATC-3′	425	66
GAPDH	5′-ATCACTGCCACCCAGAAGACT-3′	5′-ATCGAAGGTGGAAGAGTGGGA-3′	350	60
Adiponectin	5′-AGCCGCTTATGTGTATCGCT-3′	5′-GAGTCCCGGAATGTTGCAGT-3′	150	60
Osteocalcin	5′-GCAATAAGGTAGTGAACAGACTCC-3′	5′-GTTTGTAGGCGGTCTTCAAGC-3′	120	66
Aggrecan	5′-CCCTCGGGCAGAAGAAAGAT-3′	5′-CGCTTCTGTAGCCTGTGCTTG-3′	150	60
β-Actin	5’-GATGACCCAGATCATGTTTGAGA-3’	5′-GTCTCCGGAGTCCATCACAAT-3′	120	60
Telomere	5′-CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT-3′	5′-GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT-3′
36B4	5′-ACTGGTCTAGGACCCGAGAAG-3′	5′-TCAATGGTGCCTCTGGAGATT-3′