Table 2. Michaelis–Menten Parameters of Pep2-Catalyzed Phosphorylation of Maltose.
| [TreS]/[Pep2] | 0 | 1:1 | 2:1 | 4:1 |
|---|---|---|---|---|
| Maltose ([ATP] = 0.5 mM) | ||||
| Vmax (μmol min–1 mg–1) | 707 ± 50 | 1164 ± 148 | 1625 ± 399 | 2170 ± 360 |
| KM (mM) | 0.17 ± 0.04 | 0.341 ± 0.12 | 0.540 ± 0.31 | 0.457 ± 0.19 |
| kcat (sec–1) | 613.6 | 1010.4 | 1410.6 | 1883.7 |
| kcat/KM (sec–1 M–1 106) | 3.71 | 2.96 | 2.61 | 4.12 |
| ATP ([Mal] = 20 mM) | ||||
| Vmax (μmol min–1 mg–1) | 1185 ± 70 | 1904 ± 76 | 3416 ± 146 | 4164 ± 221 |
| KM (mM) | 0.031 ± 0.005 | 0.050 ± 0.005 | 0.058 ± 0.006 | 0.049 ± 0.007 |
| kcat (sec–1) | 1028.6 | 1652.8 | 2965.3 | 3614.6 |
| kcat/KM (sec–1 M–1 106) | 33.7 | 33.1 | 51.5 | 73.2 |