. Author manuscript; available in PMC: 2014 Jun 21.

Published in final edited form as: Life Sci. 2013 Apr 26;92(23):1101–1109. doi: 10.1016/j.lfs.2013.04.006

Table 2. Effects of DPDPE and SKF-82958 pretreatment on K_i values of DOPR agonists in inhibiting [³H]diprenorphine binding and on EC₅₀ and E_max values of DPDPE in stimulating [³⁵S]GTPγS binding to membranes of CHO-FLAG-DOPR/HA-D₁R cells.

See Fig. 1 legend. In addition, competitive inhibition of [³H]diprenorphine by DPDPE and SNC-80 binding was conducted and K_i values were determined. Data are expressed as mean ± s.e.m. of three independent experiments performed in duplicate.

Pretreatment	K_i (nM)		EC₅₀ (nM)	E_max (fmol/mg protein)
Pretreatment	DPDPE	SNC-80	DPDPE
Control	8.5 ± 1.2	0.83 ± 0.23	39.3 ± 6.5	48.3 ± 6.8
DPDPE	21.8 ± 5.1^*	1.11 ± 0.31	47.0 ± 10.9	14.3 ± 1.1^**
SKF	5.6 ± 0.4	0.59 ± 0.16	60.9 ± 8.9	33.4 ± 2.8^*

P<0.05,

^**

P<0.01, compared to the control by one-way ANOVA, followed by Dunnett’s t test.

Table 2. Effects of DPDPE and SKF-82958 pretreatment on Ki values of DOPR agonists in inhibiting [3H]diprenorphine binding and on EC50 and Emax values of DPDPE in stimulating [35S]GTPγS binding to membranes of CHO-FLAG-DOPR/HA-D1R cells.

Table 2. Effects of DPDPE and SKF-82958 pretreatment on K_i values of DOPR agonists in inhibiting [³H]diprenorphine binding and on EC₅₀ and E_max values of DPDPE in stimulating [³⁵S]GTPγS binding to membranes of CHO-FLAG-DOPR/HA-D₁R cells.