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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: J Agric Food Chem. 2013 Feb 17;61(8):1841–1849. doi: 10.1021/jf3033649

Figure 3.

Figure 3

Effect of a pregnane glycoside (ikemagenin) on food intake and levels of central satiety peptides in the hypothalamus. (A) Chemical structure of ikemagenin showing four saccharide moieties attached at the C3 position including oleandrose (Ole), digitoxose (Dig), and cymarose (Cym). (B) Daily food intake of male rats (n = 4) receiving a single oral dose of 10 mg/kg ikemagenin. (C) Hypothalamic mRNA expression of anorexic (POMC, CART, and BDNF) and orexigenic (AgRP and NPY) central satiety peptides in male rats (n = 4) treated with vehicle (Ctr) or a single oral dose of 10 mg/kg ikemagenin (Ike). (D) The hypothalamic AgRP protein level decreased following a single oral dose of 10 mg/kg ikemagenin as measured by Western blot analysis and was quantified densitometrically using GAPDH as a loading control. Two representative animals from vehicle (Ctr) or ikemagenin (Ike)-treated groups are shown. Asterisks indicate a significant difference (*p < 0.05) from respective controls as determined by Student’s t test.