Figure 1. Anti-HSV efficacy of HM.

[A] Plaque Reduction Assay. Infected cells were treated with HM or ACV at 0.5-50 μg/ml, overlaid with methylcellulose and plaques developed after 2-3 days were stained. The % of plaque number reduction was calculated, and the effective concentration of drug that inhibited the number of viral plaques was interpolated from the dose-response curve. [B] Time course analysis. Inhibitory effects of HM and ACV at various time points prior to infection (-3 to -1 h), at the time of infection (0 h) and post-infection (2-24 h) with HSV-2 were evaluated by plaque reduction assay. Each bar represents the mean ± S.E.M of three independent experiments.