Skip to main content
. 2013 Oct 22;8(10):e77937. doi: 10.1371/journal.pone.0077937

Figure 3. Effect of HM and ACV on HSV-2 attachment and penetration.

Figure 3

[A] Attachment assay: Prechilled cells at 4°C for 1 h were infected with HSV-2G (200 pfu) and then untreated or treated with HM. After incubating at 4°C for another 3 h, the medium was aspirated, washed and overlaid with methylcellulose to form plaques. The plaques developed after 72 h were stained and counted. [B] Penetration assay: Prechilled cells at 4°C for 1 h was infected with HSV-2G (300 pfu) for 3 h at 4°C and then untreated or treated with HM (5µg/ml) or DMSO (0.1%), and incubated for 20 min at 37°C to facilitate viral penetration. Then the extracellular non-penetrated virus was inactivated by citrate buffer (pH 3.0) for 1 min, washed with PBS and overlaid with overlay medium to form plaques. The plaques developed were then stained and counted.