Table 1. Influence of Postn on changes of tibial bone microarchitecture in response to fatigue.
|
Baseline
|
15 days after fatigue
|
30 days after fatigue
|
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|---|---|---|---|---|---|---|---|
| NON-FATIGUED | Postn+/+ | Postn-/- | Postn+/+ | Postn-/- | Postn+/+ | Postn-/- | |
| Trabecular | BV/TV (%) | 8.6 ± 1.2 | 9.9 ± 1.8 | 10.2 ± 1.2 | 9.0 ± 0.7 | 11.6 ± 0.8 | 8.9 ± 1.1 * |
| TbN (1/mm) | 3.86 ± 0.18 | 4.40 ± 0.28 | 4.18 ± 0.12 | 3.94 ± 0.17 | 4.27 ± 0.12 | 3.88 ± 0.25 | |
| TbTh (µm) | 48.0 ± 2.1 | 44.6 ± 1.5 | 48.2 ± 1.4 | 47.4 ± 1.4 | 50.0 ± 2.6 | 48.3 ± 1.6 | |
| Proximal-CorticalMidshaft-Cortical | TV (mm3) | 2.81 ± 0.08 | 2.50 ± 0.15*** | 3.31 ± 0.11 | 2.74 ± 0.10*** | 3.04 ± 0.10 | 2.60 ± 0.04*** |
| BV (mm3) | 1.25 ± 0.04 | 0.93 ± 0.04** | 1.47 ± 0.06 | 1.23 ± 0.04** | 1.26 ± 0.04 | 1.05 ± 0.02** | |
| TV (mm3) | 0.67 ± 0.02 | 0.54 ± 0.03** | 0.76 ± 0.03 | 0.57 ± 0.02** | 0.76 ± 0.05 | 0.58 ± 0.01** | |
| BV (mm3) | 0.41 ± 0.01 | 0.33 ± 0.02** | 0.46 ± 0.02 | 0.36 ± 0.01** | 0.46 ± 0.02 | 0.36 ± 0.01** | |
| FATIGUED | |||||||
| TrabecularProximal-Cortical | BV/TV (%) | 8.9 ± 1.1 | 9.8 ± 1.9 | 10.4 ± 1.1 | 9.6 ± 0.7 | 11.2 ± 0.9 | 8.6 ± 1.1 |
| TbN (1/mm) | 3.84 ± 0.12 | 4.29 ± 0.45 | 4.25 ± 0.13 | 3.94 ± 0.17 | 4.35 ± 0.14 | 3.86 ± 0.26 | |
| TbTh (µm) | 48.5 ± 1.8 | 45.7 ± 1.5 | 49.9 ± 1.2 | 48.9 ± 1.3 | 48.0 ± 1.6 | 48.9 ± 1.0 | |
| TV (mm3) | 2.82 ± 0.10 | 2.44 ± 0.16 | 3.61 ± 0.14$ | 2.80 ± 0.11 | 3.25 ± 0.10 $ | 2.77 ± 0.08 $ | |
| Midshaft-Cortical | BV (mm3) | 1.24 ± 0.05 | 0.95 ± 0.05 | 1.60 ± 0.07$ | 1.29 ± 0.05 | 1.33 ± 0.04 | 1.11 ± 0.03 |
| TV (mm3) | 0.66 ± 0.03 | 0.54 ± 0.02 | 0.74 ± 0.03 | 0.58 ± 0.02 | 0.77 ± 0.04 | 0.58 ± 0.02 | |
| BV (mm3) | 0.40 ± 0.02 | 0.33 ± 0.02 | 0.45 ± 0.02 | 0.36 ± 0.01 | 0.47 ± 0.02 | 0.37 ± 0.01 | |
Cancellous bone microarchitecture was evaluated at proximal tibia and cortical microarchitecture at midshaft tibia in the axially compressed bone and its controlateral non-stimulated control bone by in-vivo microCT at baseline, 15 days and 30 days after fatigue (n=8 mice / group). $ p<0.05 vs non-fatigued (paired t test). P value for differences between periostin groups independtly of fatigue, * p<0.05, ** p<0.01, *** p<0.001 Postn-/- vs Postn+/+. Means ± SEM.