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. 2013 Oct 22;8(10):e76088. doi: 10.1371/journal.pone.0076088

Table 1. Obs. pI and Mr, observed pI, Mr calculated from the 2D-gels with PDQuest 8.0.1 software according to standard marker proteins.

Ssp Name Accession No Protein Score Mr Theor./obs. pI Theor./obs. Expression pattern
Proteins involved in ATP generation, transport and carbohydrate metabolism
3407 ATP synthase beta subunit [Triticum aestivum] gi|525291 211 59.21/71.50 5.56/5.58 up
4018 thiazole biosynthetic enzyme [Pseudotsuga menziesii] gi|56481847 118 37.30/34.22 5.85/5.44 up
7204 glutamate-1-semialdehyde 2,1-aminomutase [Nicotiana tabacum] gi|19875 118 50.98/55.18 7.05/6.47 up
7304 isocitrate dehydrogenase (NADP+) [Arabidopsis thaliana] gi|15221788 85 47.20/65.31 7.58/6.37 up
5305 enolase2 [Zea mays] gi|162460735 182 48.13/68.08 5.70/6.03 down
3113 Cytochrome c[Ginkgo biloba] gi|117987 54 123.5/48.10 9.76/5.14 down
3109 33 kDa oxygen-evolving protein [Arabidopsis thaliana] gi|22571 142 35.11/35.08 5.68/5.31 disappear
7704 putative transketolase [Oryza sativa Japonica Group] gi|28190676 103 79.98/94.68 6.12/6.30 disappear
8104 NADPH thioredoxin reductase [Arabidopsis thaliana] gi|468524 73 32.42/38.43 5.78/6.60 disappear
Protein metabolism
3605 heat shock protein 70 like protein [Arabidopsis thaliana] gi|4467097 84 71.13/88.74 5.31/5.54 up
3611 heat shock protein 70 [Cucumis sativus] gi|6911553 123 70.00/92.07 5.29/5.63 up
8408 26S proteasome ATPase subunit [Pisum sativum] gi|49175787 214 23.56/68.35 7.16/6.69 up
4207 Protein disulfide-isomerase [Medicago truncatula] gi|357442333 80 40.47/55.98 5.54/5.81 down
4312 Elongation factor Tu [Chlamydomonas reinhardtii] gi|41179007 182 45.71/61.41 5.90/5.89 down
5313 Elongation factor Tu [Arabidopsis thaliana] gi|15236220 112 49.38/62.11 6.25/6.18 down
ROS scavenging and detoxifying enzymes
5004 GST [Ginkgo biloba] gi|66736578 421 25.77/26.64 6.24/5.71 up
6008 ascorbate peroxidase [Ginkgo biloba] gi|220898265 296 27.51/30.31 5.81/6.29 down
9111 ferredoxin-NADP + reductase [Arabidopsis thaliana] gi|162459168 98 39.30/40.13 8.53/8.66 up
Wall remodeling and metabolism
7110 2-dehydro-3-deoxyphosphooctonate aldolase [Arabidopsis thaliana] gi|13620976 65 31.60/37.81 6.33/6.38 up
2207 golgi associated protein se- Zea mays] gi|162463414 124 41.18/57.79 5.75/5.38 down
Transcription, cell cycle and signal transduction
4607 ARF-L1 protein [Ginkgo biloba] gi|291196881 40 110.7/89.10 5.64/5.45 up
4801 Cell division cycle protein 48 homolog gi|1705678 69 89.77/100.8 5.18/5.72 up
8505 putative polyprotein [Nicotiana tabacum] gi|15963359 65 61.40/85.59 9.36/6.51 up
Unclassified proteins
3206 Os02g0698000 [Oryza sativa (japonica cultivar-group)] gi|115448091 75 44.84/54.23 5.68/5.14 down
5102 putative protein [Arabidopsis thaliana] gi|7573371 98 34.75/47.97 6.19/6.00 down
6409 Os02g0125100 [Oryza sativa (japonica cultivar-group)] gi|115443927 73 55.50/75.83 7.05/6.15 up
6512 Sequence 4 from patent US 6946283 gi|77372606 40 99.47/77.53 5.53/6.36 down

ER: endoplasmic reticulum, PM: Plasma Membrane, M: Mitochondrion.