Table 1. Obs. pI and Mr, observed pI, Mr calculated from the 2D-gels with PDQuest 8.0.1 software according to standard marker proteins.
Ssp | Name | Accession No | Protein Score | Mr Theor./obs. | pI Theor./obs. | Expression pattern |
Proteins involved in ATP generation, transport and carbohydrate metabolism | ||||||
3407 | ATP synthase beta subunit [Triticum aestivum] | gi|525291 | 211 | 59.21/71.50 | 5.56/5.58 | up |
4018 | thiazole biosynthetic enzyme [Pseudotsuga menziesii] | gi|56481847 | 118 | 37.30/34.22 | 5.85/5.44 | up |
7204 | glutamate-1-semialdehyde 2,1-aminomutase [Nicotiana tabacum] | gi|19875 | 118 | 50.98/55.18 | 7.05/6.47 | up |
7304 | isocitrate dehydrogenase (NADP+) [Arabidopsis thaliana] | gi|15221788 | 85 | 47.20/65.31 | 7.58/6.37 | up |
5305 | enolase2 [Zea mays] | gi|162460735 | 182 | 48.13/68.08 | 5.70/6.03 | down |
3113 | Cytochrome c[Ginkgo biloba] | gi|117987 | 54 | 123.5/48.10 | 9.76/5.14 | down |
3109 | 33 kDa oxygen-evolving protein [Arabidopsis thaliana] | gi|22571 | 142 | 35.11/35.08 | 5.68/5.31 | disappear |
7704 | putative transketolase [Oryza sativa Japonica Group] | gi|28190676 | 103 | 79.98/94.68 | 6.12/6.30 | disappear |
8104 | NADPH thioredoxin reductase [Arabidopsis thaliana] | gi|468524 | 73 | 32.42/38.43 | 5.78/6.60 | disappear |
Protein metabolism | ||||||
3605 | heat shock protein 70 like protein [Arabidopsis thaliana] | gi|4467097 | 84 | 71.13/88.74 | 5.31/5.54 | up |
3611 | heat shock protein 70 [Cucumis sativus] | gi|6911553 | 123 | 70.00/92.07 | 5.29/5.63 | up |
8408 | 26S proteasome ATPase subunit [Pisum sativum] | gi|49175787 | 214 | 23.56/68.35 | 7.16/6.69 | up |
4207 | Protein disulfide-isomerase [Medicago truncatula] | gi|357442333 | 80 | 40.47/55.98 | 5.54/5.81 | down |
4312 | Elongation factor Tu [Chlamydomonas reinhardtii] | gi|41179007 | 182 | 45.71/61.41 | 5.90/5.89 | down |
5313 | Elongation factor Tu [Arabidopsis thaliana] | gi|15236220 | 112 | 49.38/62.11 | 6.25/6.18 | down |
ROS scavenging and detoxifying enzymes | ||||||
5004 | GST [Ginkgo biloba] | gi|66736578 | 421 | 25.77/26.64 | 6.24/5.71 | up |
6008 | ascorbate peroxidase [Ginkgo biloba] | gi|220898265 | 296 | 27.51/30.31 | 5.81/6.29 | down |
9111 | ferredoxin-NADP + reductase [Arabidopsis thaliana] | gi|162459168 | 98 | 39.30/40.13 | 8.53/8.66 | up |
Wall remodeling and metabolism | ||||||
7110 | 2-dehydro-3-deoxyphosphooctonate aldolase [Arabidopsis thaliana] | gi|13620976 | 65 | 31.60/37.81 | 6.33/6.38 | up |
2207 | golgi associated protein se- Zea mays] | gi|162463414 | 124 | 41.18/57.79 | 5.75/5.38 | down |
Transcription, cell cycle and signal transduction | ||||||
4607 | ARF-L1 protein [Ginkgo biloba] | gi|291196881 | 40 | 110.7/89.10 | 5.64/5.45 | up |
4801 | Cell division cycle protein 48 homolog | gi|1705678 | 69 | 89.77/100.8 | 5.18/5.72 | up |
8505 | putative polyprotein [Nicotiana tabacum] | gi|15963359 | 65 | 61.40/85.59 | 9.36/6.51 | up |
Unclassified proteins | ||||||
3206 | Os02g0698000 [Oryza sativa (japonica cultivar-group)] | gi|115448091 | 75 | 44.84/54.23 | 5.68/5.14 | down |
5102 | putative protein [Arabidopsis thaliana] | gi|7573371 | 98 | 34.75/47.97 | 6.19/6.00 | down |
6409 | Os02g0125100 [Oryza sativa (japonica cultivar-group)] | gi|115443927 | 73 | 55.50/75.83 | 7.05/6.15 | up |
6512 | Sequence 4 from patent US 6946283 | gi|77372606 | 40 | 99.47/77.53 | 5.53/6.36 | down |
ER: endoplasmic reticulum, PM: Plasma Membrane, M: Mitochondrion.