Figure 5. Effect of pyruvate carboxylase shRNA interference on the regulation of geniposide on the uptake and metabolism of glucose in INS-1 cells.

A: The interfering efficiency of shRNA for pyruvate carboxylase gene was determined by western blot. B: Interference on pyruvate carboxylase expression reversed the influence of geniposide on glucose uptake in the presence of low or high concentration of glucose. C: Interference on pyruvate carboxylase expression inhibited the effect of geniposide on ATP production in the presence of low or high concentration of glucose. Data are means ± SD from three representative experiments.