Table 2. Summary of embryo microinjection of ZFN mRNA and ssODN.

ZFN mRNA	ssDNA	Embryos injected	Embryos transferred	Recipient	Pups	Pups with Bgl II or loxP site	Precise RH Rate	No. pups
5 ng/μl each	0.5 μM Bgl II-50H	260	208	8	13	1	0 (0/13)	#4-1 to #4-8, #5-1 to #5-5
5 ng/μl each	1 μM Bgl II-50H	341	257	7	16	2	6.25% (1/16)	#1-1 to #1-2, #2-1 to #2-3, #3-1 to #3-11
5 ng/μl each	0.5 μM Bgl II-100H	305	213	7	69	5	7.25% (5/69)	A1-A35, B1-B21, C1-C13
5 ng/μl each	1 μM Bgl II-100H	241	211	7	11	2	18.18% (2/11)	#6-1 to #6-4, #7-1 to #7-7
5 ng/μl each	1 μM loxP-80H	120	52	2	9	2	22.22% (2/9)	54-62