Table 2. Summary of embryo microinjection of ZFN mRNA and ssODN.
ZFN mRNA | ssDNA | Embryos injected | Embryos transferred | Recipient | Pups | Pups with Bgl II or loxP site | Precise RH Rate | No. pups |
---|---|---|---|---|---|---|---|---|
5 ng/μl each | 0.5 μM Bgl II-50H | 260 | 208 | 8 | 13 | 1 | 0 (0/13) | #4-1 to #4-8, #5-1 to #5-5 |
5 ng/μl each | 1 μM Bgl II-50H | 341 | 257 | 7 | 16 | 2 | 6.25% (1/16) | #1-1 to #1-2, #2-1 to #2-3, #3-1 to #3-11 |
5 ng/μl each | 0.5 μM Bgl II-100H | 305 | 213 | 7 | 69 | 5 | 7.25% (5/69) | A1-A35, B1-B21, C1-C13 |
5 ng/μl each | 1 μM Bgl II-100H | 241 | 211 | 7 | 11 | 2 | 18.18% (2/11) | #6-1 to #6-4, #7-1 to #7-7 |
5 ng/μl each | 1 μM loxP-80H | 120 | 52 | 2 | 9 | 2 | 22.22% (2/9) | 54-62 |