Figure 1. Gene expression by fetal LSECs. CD31 expression on CD14⁺⁺ cells measured by flow cytometry and mRNA levels.

(A). Viable non-hematopoietic cells (PI⁻CD45⁻) gated by CD326 and CD14 expression (a: CD14⁻CD326⁻; b: CD326^loCD14⁻; c: CD14⁺⁺CD326^+/−; d: CD326⁺⁺CD14^lo). Expression of CD31 was analyzed by qPCR in each sorted population (n = 3). Flow cytometric plots shows expression of CD31 versus CD326 and CD31 versus CD14. (B): Gene expression profile of sorted populations (a: CD326^loCD14⁻; b: CD14⁺⁺CD326⁻; c: CD14⁺⁺CD326⁺; d: CD326⁺⁺CD14^lo) measured by qPCR, n = 7.