Figure 7. Culture of human cells recovered from the livers of transplanted uPA-NOG mice.

Livers were enzymatically digested and light density cells isolated, which were cultured on collagen-coated plates with EBM2 supplied medium. Cells became confluent after 3 weeks, forming a cobble-stone layer typical for cultured human endothelial cells. The cells expressed human CD31 (green) and were negative for staining with mouse H-2Kd (not shown). Nuclei are stained with DAPI (blue).