Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Brain Res Bull. 2013 Jul 1;98:23–29. doi: 10.1016/j.brainresbull.2013.06.005

Fig. 1. Isoflurane preconditioning-induced neuroprotection.

Fig. 1

Mice were pretreated with or without 1.5% isoflurane for 30 min at 60 min before a 90-min middle cerebral arterial occlusion (MCAO). The results were evaluated at 24 h after the MCAO. A: Brain slices stained with 2,3,5-triphenyltetrazolium chloride from representative mice. B, C and D: Percentage of total, cortical and subcortical infarct volume in ipsilateral hemisphere volume. Results are the means ± S.D. (n = 10 – 11). E: Neurological deficit scores evaluated immediately before the animals were euthanized for the assessment of infarct sizes (data are presented in panels B, C and D) or assigned 7 to the animals that died before the end of observation period. Results are presented in a box plot format (n = 10 – 11). ●: lowest or highest score (the score will not show up if it falls in the 95% interval); between lines: 95% interval of the data; inside boxes: 25–75% interval including the median of the data. F: The performance on rotarod. Rats were tested before and 24 h after the MCAO and the speed-latency index ratio of these two tests are presented. Results are the means ± S.D. (n = 10 – 11). * P < 0.05 compared with the wildtype mice subjected to MCAO only. ^ P < 0.05 compared with wild-type mice subjected to isoflurane preconditioning plus MCAO. Iso: isoflurane; LY: LY294004; EAAT3−/−: EAAT3 knockout.