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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Aug 13;272(3):780–786. doi: 10.1016/j.taap.2013.08.002

Figure 2. Effect of pregnenolone and mono-OH MXC co-treatment on levels of androstenedione and testosterone in media from mouse antral follicles.

Figure 2

Isolated mouse antral follicles were cultured for 96 h with DMSO as a vehicle control, mono-OH MXC (10 μg/mL), pregnenolone (1 μg/mL), or mono-OH MXC and pregnenolone together. Following culture, media samples from individual follicles were subjected to measurement of androstenedione and testosterone levels as described in Materials and Methods. Data were expressed as mean ± SE calculated from follicles in three separate experiments. Data for testosterone levels were log-transformed to achieve normality. Different letters indicate differences between treatment groups (p<0.05).